Severe acute respiratory syndrome coronavirus nucleocapsid protein expressed by an adenovirus vector is phosphorylated and immunogenic in mice
Identifieur interne : 004D64 ( Main/Exploration ); précédent : 004D63; suivant : 004D65Severe acute respiratory syndrome coronavirus nucleocapsid protein expressed by an adenovirus vector is phosphorylated and immunogenic in mice
Auteurs : Alexander N. Zakhartchouk [Canada] ; Sathiyanarayanan Viswanathan [Canada] ; James B. Mahony [Canada] ; Jack Gauldie [Canada] ; Lorne A. Babiuk [Canada]Source :
- Journal of general virology [ 0022-1317 ] ; 2005.
Descripteurs français
- KwdFr :
- Adenoviridae (génétique), Adenoviridae (métabolisme), Animaux, Anticorps antiviraux (biosynthèse), Femelle, Lymphocytes T (immunologie), Masse moléculaire, Phosphorylation, Protéines nucléocapside (biosynthèse), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Protéines recombinantes (biosynthèse), Souris, Souris de lignée C57BL, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (immunologie), Vaccination (), Vaccins antiviraux (biosynthèse), Vaccins antiviraux (génétique), Vaccins antiviraux (immunologie), Vecteurs génétiques, Virus du SRAS (génétique), Virus du SRAS (immunologie), Évaluation préclinique de médicament.
- MESH :
- biosynthèse : Anticorps antiviraux, Protéines nucléocapside, Protéines recombinantes, Vaccins antiviraux.
- génétique : Adenoviridae, Protéines nucléocapside, Vaccins antiviraux, Virus du SRAS.
- immunologie : Lymphocytes T, Protéines nucléocapside, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Virus du SRAS.
- métabolisme : Adenoviridae.
- Pascal (Inist)
- Animaux, Coronavirus, Adenoviridae, Femelle, Masse moléculaire, Phosphorylation, Souris, Grave, Malade état grave, Aigu, Souris de lignée C57BL, Syndrome respiratoire aigu sévère, Vaccination, Vecteurs génétiques, Voie respiratoire, Nucléocapside, Protéine, Vecteur, Phosphorylation, Syndrome respiratoire aigu sévère, Immunogénicité, Animal, Microbiologie, Virologie, Forme grave, Évaluation préclinique de médicament.
English descriptors
- KwdEn :
- Acute, Adenoviridae, Adenoviridae (genetics), Adenoviridae (metabolism), Animal, Animals, Antibodies, Viral (biosynthesis), Coronavirus, Critically ill, Drug Evaluation, Preclinical, Female, Genetic Vectors, Immunogenicity, Mice, Mice, Inbred C57BL, Microbiology, Molecular Weight, Mouse, Nucleocapsid, Nucleocapsid Proteins (biosynthesis), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Phosphorylation, Protein, Recombinant Proteins (biosynthesis), Respiratory tract, SARS Virus (genetics), SARS Virus (immunology), Severe, Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (prevention & control), Severe acute respiratory syndrome, T-Lymphocytes (immunology), Vaccination (methods), Vector, Viral Vaccines (biosynthesis), Viral Vaccines (genetics), Viral Vaccines (immunology), Virology.
- MESH :
- chemical , biosynthesis : Antibodies, Viral, Nucleocapsid Proteins, Recombinant Proteins, Viral Vaccines.
- genetics : Adenoviridae, Nucleocapsid Proteins, SARS Virus, Viral Vaccines.
- chemical , immunology : Nucleocapsid Proteins, SARS Virus, Severe Acute Respiratory Syndrome, T-Lymphocytes, Viral Vaccines.
- metabolism : Adenoviridae.
- methods : Vaccination.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Drug Evaluation, Preclinical, Female, Genetic Vectors, Mice, Mice, Inbred C57BL, Molecular Weight, Phosphorylation.
Abstract
Severe acute respiratory syndrome coronavirus (SARS-CoV) has been identified as the aetiological agent of SARS. Thus, vaccination against SARS-CoV may represent an effective approach towards controlling SARS. The nucleocapsid (N) protein is thought to play a role in induction of cell-mediated immunity to SARS-CoV and thus it is important to characterize this protein. In the present study, an E1/partially E3-deleted, replication-defective human adenovirus 5 (Ad5) vector (Ad5-N-V) expressing the SARS-CoV N protein was constructed. The N protein, expressed in vitro by Ad5-N-V, was of the expected molecular mass of 50 kDa and was phosphorylated. Vaccination of C57BL/6 mice with Ad5-N-V generated potent SARS-CoV-specific humoral and T cell-mediated immune responses. These results show that Ad5-N-V may potentially be used as a SARS-CoV vaccine.
Affiliations:
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Zakhartchouk</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Vaccine and Infectious Disease Organization, University of Saskatchewan</s1><s2>Saskatoon, SK, S7N 5E3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, SK, S7N 5E3</wicri:noRegion></affiliation></author><author><name sortKey="Viswanathan, Sathiyanarayanan" sort="Viswanathan, Sathiyanarayanan" uniqKey="Viswanathan S" first="Sathiyanarayanan" last="Viswanathan">Sathiyanarayanan Viswanathan</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Vaccine and Infectious Disease Organization, University of Saskatchewan</s1><s2>Saskatoon, SK, S7N 5E3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, SK, S7N 5E3</wicri:noRegion></affiliation></author><author><name sortKey="Mahony, James B" sort="Mahony, James B" uniqKey="Mahony J" first="James B." last="Mahony">James B. 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Babiuk</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Vaccine and Infectious Disease Organization, University of Saskatchewan</s1><s2>Saskatoon, SK, S7N 5E3</s2><s3>CAN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>5 aut.</sZ></inist:fA14><country>Canada</country><wicri:noRegion>Saskatoon, SK, S7N 5E3</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of general virology</title><title level="j" type="abbreviated">J. gen. virol.</title><idno type="ISSN">0022-1317</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of general virology</title><title level="j" type="abbreviated">J. gen. virol.</title><idno type="ISSN">0022-1317</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acute</term><term>Adenoviridae</term><term>Adenoviridae (genetics)</term><term>Adenoviridae (metabolism)</term><term>Animal</term><term>Animals</term><term>Antibodies, Viral (biosynthesis)</term><term>Coronavirus</term><term>Critically ill</term><term>Drug Evaluation, Preclinical</term><term>Female</term><term>Genetic Vectors</term><term>Immunogenicity</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Microbiology</term><term>Molecular Weight</term><term>Mouse</term><term>Nucleocapsid</term><term>Nucleocapsid Proteins (biosynthesis)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>Phosphorylation</term><term>Protein</term><term>Recombinant Proteins (biosynthesis)</term><term>Respiratory tract</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Severe</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Severe acute respiratory syndrome</term><term>T-Lymphocytes (immunology)</term><term>Vaccination (methods)</term><term>Vector</term><term>Viral Vaccines (biosynthesis)</term><term>Viral Vaccines (genetics)</term><term>Viral Vaccines (immunology)</term><term>Virology</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adenoviridae (génétique)</term><term>Adenoviridae (métabolisme)</term><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Femelle</term><term>Lymphocytes T (immunologie)</term><term>Masse moléculaire</term><term>Phosphorylation</term><term>Protéines nucléocapside (biosynthèse)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines recombinantes (biosynthèse)</term><term>Souris</term><term>Souris de lignée C57BL</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Vaccination ()</term><term>Vaccins antiviraux (biosynthèse)</term><term>Vaccins antiviraux (génétique)</term><term>Vaccins antiviraux (immunologie)</term><term>Vecteurs génétiques</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Évaluation préclinique de médicament</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Vaccins antiviraux</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Adenoviridae</term><term>Nucleocapsid Proteins</term><term>SARS Virus</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Adenoviridae</term><term>Protéines nucléocapside</term><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Lymphocytes T</term><term>Protéines nucléocapside</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>T-Lymphocytes</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Adenoviridae</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Vaccination</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adenoviridae</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Drug Evaluation, Preclinical</term><term>Female</term><term>Genetic Vectors</term><term>Mice</term><term>Mice, Inbred C57BL</term><term>Molecular Weight</term><term>Phosphorylation</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Animaux</term><term>Coronavirus</term><term>Adenoviridae</term><term>Femelle</term><term>Masse moléculaire</term><term>Phosphorylation</term><term>Souris</term><term>Grave</term><term>Malade état grave</term><term>Aigu</term><term>Souris de lignée C57BL</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccination</term><term>Vecteurs génétiques</term><term>Voie respiratoire</term><term>Nucléocapside</term><term>Protéine</term><term>Vecteur</term><term>Phosphorylation</term><term>Syndrome respiratoire aigu sévère</term><term>Immunogénicité</term><term>Animal</term><term>Microbiologie</term><term>Virologie</term><term>Forme grave</term><term>Évaluation préclinique de médicament</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome coronavirus (SARS-CoV) has been identified as the aetiological agent of SARS. Thus, vaccination against SARS-CoV may represent an effective approach towards controlling SARS. The nucleocapsid (N) protein is thought to play a role in induction of cell-mediated immunity to SARS-CoV and thus it is important to characterize this protein. In the present study, an E1/partially E3-deleted, replication-defective human adenovirus 5 (Ad5) vector (Ad5-N-V) expressing the SARS-CoV N protein was constructed. The N protein, expressed in vitro by Ad5-N-V, was of the expected molecular mass of 50 kDa and was phosphorylated. Vaccination of C57BL/6 mice with Ad5-N-V generated potent SARS-CoV-specific humoral and T cell-mediated immune responses. These results show that Ad5-N-V may potentially be used as a SARS-CoV vaccine.</div></front></TEI><affiliations><list><country><li>Canada</li></country><region><li>Ontario</li></region><settlement><li>Hamilton (Ontario)</li></settlement><orgName><li>Université McMaster</li></orgName></list><tree><country name="Canada"><noRegion><name sortKey="Zakhartchouk, Alexander N" sort="Zakhartchouk, Alexander N" uniqKey="Zakhartchouk A" first="Alexander N." last="Zakhartchouk">Alexander N. Zakhartchouk</name></noRegion><name sortKey="Babiuk, Lorne A" sort="Babiuk, Lorne A" uniqKey="Babiuk L" first="Lorne A." last="Babiuk">Lorne A. Babiuk</name><name sortKey="Gauldie, Jack" sort="Gauldie, Jack" uniqKey="Gauldie J" first="Jack" last="Gauldie">Jack Gauldie</name><name sortKey="Mahony, James B" sort="Mahony, James B" uniqKey="Mahony J" first="James B." last="Mahony">James B. Mahony</name><name sortKey="Mahony, James B" sort="Mahony, James B" uniqKey="Mahony J" first="James B." last="Mahony">James B. Mahony</name><name sortKey="Viswanathan, Sathiyanarayanan" sort="Viswanathan, Sathiyanarayanan" uniqKey="Viswanathan S" first="Sathiyanarayanan" last="Viswanathan">Sathiyanarayanan Viswanathan</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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